It comes after a tumultuous few months for the San Francisco start-up that led to former CEO and co-founder Travis Kalanick being forced out after a series of boardroom controversies.
TfL said on Friday it would not comment before the licensing appeal hearing due later this year.
Only a month into the job, Chief Executive Dara Khosrowshahi met TfL Commissioner Mike Brown for talks earlier in October, which both sides said were constructive as the $70-billion firm tries to repair its relationship with the regulator.
A spokesman at the Mayor of London’s office said that Khan, a politician from the opposition Labour Party who is also chairman of TfL, continued to back the license decision and that all private hire firms must play by the rules.
The filing is a short notification of Uber’s intention to appeal rather than the detailed grounds.
London’s decision is one of the most serious setbacks to the taxi app, which has been forced to quit several countries, including Denmark and Hungary, and faced regulatory battles in multiple U.S. states and around the world.